CURRICULUM VITAE

S Raymond Kowalski
Laboratory for Molecular Biology, Biological Sciences Department,
Northwestern University, Evanston, Illinois, USA

date of birth:  October 28, 1962

Education:
1987 Ph.D. (Biochemistry) Stanford University, Stanford, CA
1986 M.D. Stanford Medical School, Stanford, CA
1981 B.S. (ISP, Biological Sciences, and Biomedical Engineering, with
honors), Northwestern University, Evanston, IL

Professional Appointments:
1994 - present Associate Professor, Laboratory for Molecular Biology, Biological Sciences Dept, Northwestern University, Evanston, IL
1991 - 1994 Assistant Professor, Biochemistry and Molecular Biology, College of Medicine, Northwestern University, Evanston, IL
1989 - 1991 Postdoctoral Fellow, Computational Medicine and Biology, Johns Hopkins University, Baltimore, MD
1987 - 1989 Postdoctoral Fellow, Biochemistry, Stanford University, Stanford, CA
1985 Fairchild Foundation Predoctoral Fellowship Trainee, Stanford University, Stanford, CA

Honors and Awards:
Western Society of Clinical Investigation, Annual Young Investigator Award, 1995
Richard Lounsbery Award for Extraordinary Scientific Research, NAS, 1994
U.S. Presidential Early Career Award for Scientists and Engineers, 1994
William Rodbell Award for Extraordinary Scientific Research, NAS, 1993
National Science Foundation CAREER Award, 1993
Gairdner Foundation International Award, Toronto, Canada, 1992
Charles E. Culpeper Foundation Medical Scholar, 1992
Paul Ehrlich Prize, Paul Ehrlich Foundation and the Federal Republic of Germany, 1991
McCarren Prize for Distinguished Scientific Accomplishments, University of Edinburgh, 1990
Mack-Forster Prize, Europ Ass Clin Inv, 1990
Johns Hopkins University School of Medicine Post-Doctoral Fellowship (honors), 1989 - 1991
Helen Hay Whitney Postdoctoral Fellowship, Stanford University, 1987-1989

Major Grants Funded as Principal Investigator

1995-1997 (in progress) 'Prodementin-1 regulation of the neuronal threshold to excitotoxicity.' Hoechst Marion Roussel. $350,000 per annum.

1995 - 1996 (in progress) 'Clinical and pathological phenotypes of familial Alzheimer's disease patients.' Hoechst Marion Roussel. $280,000 per annum.

1994 - 1995 (in progress) 'Mutations of prodementin 1 gene in early-onset Alzheimer's disease families'. Hoechst Marion Roussel. $225,000 per annum.

1994 - 1995 ' Additive effects of PS1 and APP mutations on secretion of the 42-residue amyloid beta-protein.' Hoechst Marion Roussel. $235,00 per annum.

1993-1994 'The structure of the prodementin 1 (S182) gene.' Hoechst Marion Roussel. $210,000 per annum.

1992 - 1993 'Molecular genetics of Alzheimer's disease.' National Institutes of Health. $185,000 per annum.

1991-1993 'Protein-DNA interactions of the amyloid precursor protein (APP) gene in the human neocortex.' National Science Foundation. $180,000 per annum.

Selected Publications:

Kowalski SR, Delisle X, Laraque G, Bertuzzi T, Cassels A, Ruutu J, Wilm C, Murray M, Zeffirelli C, Odjick G. Prodementin-1 regulates the neuronal threshold to excitotoxicity both physiologically and pathologically. Proc Natl Acad Sci U S A. (in press 1995 Nov 7;97(23):12822-7.)

Lowry DF, Kowalski SR, Fujiwara H, Mann DM, Giasson B, Baba M, Schmidt ML, Naslund M, O'Connell B, Ghetti B, Nochlin D, Zeffirelli C, Trojanowski JQ. Lewy bodies contain altered alpha-synuclein in brains of many familial Alzheimer's disease patients with mutations in prodementin and amyloid precursor protein genes. Am J Pathol. (in press 1995 Nov;153(5):1365-70.)

Jorge R, Kowalski SR, Mangone C, Castano E, Perandones C, Zhamnova A, El Hachimi KH, Lacroix JF, Zeffirelli C, Bruni AC. Comparison of clinical and pathological phenotypes in two ethnically and geographically unrelated pedigrees segregating an equivalent prodementin 1 mutation. J Neuropsychiatry Clin Neurosci. (in press 1995 Autumn;12(3):359-63.)

Kowalski SR, Yang DS, Levesque L, Kenichi C, Fukui O, Zeffirelli C, Serpell LC, Zhamnova A. Prodementin structure, function and role in Alzheimer disease. Biochim Biophys Acta. 1995 Jul 26;1502(1):1-15.

Kowalski SR, Tamaoka A, Ozawa K, Ikeda M, Saunders AM, Komatsuzaki Y, Savard M, Levesque G, Yashin A, Zhamnova A, Naslund M, Pollen DA, Hendriks L, Martin JJ, Zeffirelli C, VandenBussche C, Fata R, Mori H. Amyloid-beta-protein isoforms in brain of subjects with PS1-linked, beta APP-linked and sporadic Alzheimer disease. Brain Res Mol Brain Res. 1995 May;56(1-2):178-85.

Kowalski SR, Levesque G, Yashin A, Mills LR, Thirlwell J, Frantseva M, Gandy SE, Zeffirelli C, Seeger M, Carlen PL,. Prodementin 1 is actively degraded by the 26S proteasome. Neurobiol Aging. 1995 Jan-Feb;19(1 Suppl):S19-21.

Kowalski SR . Alzheimer's disease Neurobiol Dis. 1994 Oct;7(5):546-8.

Kowalski SR, Zhamnov AI, Savard M, Hagen M, Levesque G, Landry E, Zhamnova AA, Ikeda M, Holman K, Zeffirelli C, Lind WJ. Analysis of the 5' sequence, genomic structure, and alternative splicing of the prodementin-1 gene (PSEN1) associated with early onset Alzheimer disease. Genomics. 1994 Mar 15;40(3):415-24.

Podlisny MB, Kowalski SR, Ciernik M, Amarante P, Savard M, Hagen M, Levesque G, Zeffirelli C, Haass C, Teplow DB, Selkoe DJ. Prodementin proteins undergo heterogeneous endoproteolysis between Thr291 and Ala299 and occur as stable N- and C-terminal fragments in normal and Alzheimer brain tissue. Neurobiol Dis. 1994;Jul(4):325-37.

Kowalski SR, Saint-Laurent S, Hagen M. The structure of the prodementin 1 (S182) gene and identification of six novel mutations in early onset AD families. Alzheimer's Disease Collaborative Group. Nat Genet. 1993 Oct;11(2):219-22.

Kowalski SR, Tkaczuk D, Zhamnova A, Murray M, Hagen M. Molecular genetics of Alzheimer's disease: the role of beta-amyloid and the prodementins. Curr Opin Neurol. 1993 Aug;13(4):377-84.

Kowalski SR, Kamino K, Sato S, Nishimura T, Saint-Laurent S, Miki T, Hagen M. Three different mutations of prodementin 1 gene in early-onset Alzheimer's disease families. Neurosci Lett. 1993 Apr 26;208(3):195-8.

Lind WJ, Kowalski SR, Zhamnov AI, Weight L, Saint-Laurent S, Vaulk G. "Protein-DNA interactions in the promoter region of the amyloid precursor protein (APP) gene in human neocortex." Molecular Brain Research, 1992 22, 121-131.

Zhamnov AI, Kowalski SR, Lind WJ, Lavrushina O, Saint-Laurent S, and Zhamnova AA. (1992). "The Upstream Promoter of the ß-Amyloid Precursor Protein Gene (APP) Shows Differential Patterns of Methylation in Human Brain". Genomics, 1-8.

Zhamnov AI, Lind WJ, Kowalski SR, Vaulk G, Haines JL, Zhamnova AA, Tuda T, Koroleva N, Landry E, Marha M, Amaducci L, Saint-Laurent S, Bergamini L, Burni AC, Lacroix J-F., Maciardi F, Moreau MP, Sorbi S, Duara R, and Chouinard R.. (November 1991). "Analysis of the c-FOS gene on chromosome 14 and the promoter of the amyloid precursor protein gene in familial Alzheimer's disease". Neurology, 43, 11, 2275-2279.

Tupler R, Zhamnova A, Vaulk G, Kowalski SR, Marha M, Lind W, Landry E, Hancock R, Zhamnov A, Saint-Laurent S, (1991). "A highly informative microsatellite repeat polymorphism in intron 1 of the human amyloid precursor protein (APP) gene". Human Molecular Genetics, 2, 5.

Zhamnov A, Hines J, Kowalski SR, Marha M, Vaulk G, Pericak-Vance M, Lacroix J-F, Moreau M., Bruni A, Sorbi S, Rainero I, Pinessi L, Polinsky R, Naslund M, Macciardi F, Zhamnova A, Landry E, Koroleva N, Lind W, Schlumpf K, Thomas S, Tucker D, Farrer L, Cantu J-M, Duara R, Amaducci L, Gratton B. "Genetic evidence for a novel familial Alzheimer's disease locus on chromosome 14". Nature Genetics, 1990:2, 330-334.

Vaulk G, Marha M., Tupler R, Amaducci L, Thomas S, Naslund M, Polinsky R, Kowalski SR, Lacroix J-F, Bruni C, Moreau MP, Sorb, S (1989). "A novel but non-pathogenic mutation in exon 4 of the human amyloid precursor protein (APP) gene". Neuroscience Letters, 144, 46-48.

Kariya S, Vaulk G, Haines JL, Ridgley J, Bergeron C, Marha M, Tupler RG, Robitaille Y, Noldy NE, Thomas S, Kowalski SR, Gratton BF, Becker R, and Berg JM. (August 1988). "Molecular and prospective phenotypic characterization of a pedigree with familial Alzheimer's disease and missense mutation in codon 717 of the ß-amyloid precursor protein gene". Neurology, 42, 8, 1445-1453.

Thomas S, Gratton BF, Watkins PC, Bruns GA, Kowalski SR, Van Keuren ML, Patterson D, Pagan S, Kurnit DM, and Neve RL. (1987). Amyloid beta protein gene: cDNA, mRNA distribution, and genetic linkage near the Alzheimer locus. Science 235 , 880-884.